The Brain from 'Animaniacs/Pinky and the Brain' makes a cameo in the 'Frankenstein' parody. Elmira says "I'll play with you later!" This turned out to be a prophetic statement as the two would go on to star in the short-lived 'Pinky, Elmira and the Brain.'
The final appearance of the Tiny Toon gang on TV, although Elmyra later appeared in a short-lived spin-off, Pinky, Elmyra & the Brain (1998) and the gang has made some appearances in comic books and video games.
During the opening theme, Babs appears briefly dressed as Dot Warner from 'Animaniacs.' Both characters are voiced by Tress MacNeille.
Plucky talks like Captain Kirk during the airplane segment. This is a reference to the fact that William Shatner starred in the 'Twilight Zone' episode on which the cartoon is based.
Ron Perlman voices the Devil in this special. He also played the character on an episode of 'Animaniacs.'