At 34:40, Chase is shown drawing a circle around -Low Sugar In CSF on a whiteboard. However the marker he uses is likely low on ink and the circle is incomplete. In the next shot House walks over to the board and despite no one touching it the circle has been re-drawn to make it complete.
When Dr. Sebastian looks at the pills, there are four in the little glass, but when he pours them on the table after a cut there are only three pills, also in a different order. When he pours the last two pills, the order is pink pill, the last two pills, blue pill, but you can also see him pouring pink, blue and the two other pills. When the camera shows Sebastian and the table, the order is: pink, white, blue. After that the order of the pills is messed up again.
When House is leaving after messing up Sebastian's room, he is on the left side of the screen walking away. In the next shot he is looking back at Cameron and walking away again.
When Dr. House jokes about how Dr. Sebastian should receive the Nobel Peace Prize he mentions traveling to Stockholm, Sweden. Contrary to the other Nobel Prizes, the Peace Prize is actually awarded in Oslo, Norway.
When Dr Sebastian places the pills on the table, he drops one pills saying streptomycin. for TB, streptomycin doesn't come in pills instead inject-able form is present.
When Dr. Sebastian Charles is walking around the hospital (several scenes) he is barefoot. This is unsanitary, as he could pick up, or spread infection, and would not be allowed.