At the beginning of the episode, we see a telegram that Pinky has received. This telegram mentions a fictional place in Vienna: "Rueggerstrasse", which is a play-on of the name of Tom Ruegger, the senior producer of most of the "Pinky and the Brain" episodes.
When Trudy asks Pinky "What do you know?!" Pinky replies, "The lyrics to 'Yakko's World'." Pinky and Yakko are both voiced by Rob Paulsen.
'The Third Mouse' is a a spoof of the film 'The Third Man'.