The full first scene is of course a homage to the Frankenstein movies, with the lightning storm, Megatron acting as the evil scientist, Waspinator as Igor, and the newly born Dinobot II playing the part of Frankenstein's monster.
Before Depth Charge emerges out of the healing chamber, in the closeup of the chamber's read-out screen, the words "FFUCK YOU" flash briefly, turned upside down, and written in Cybertronix (Transformers writing). Such gags were quite frequent in the cartoon.