This is the first time that Mesprit, Azelf, and Uxie appear in their physical forms. They previously appeared as projected souls in Setting Off! From Futaba Town to Masago Town!! (2006), Pruning a Passel of Pals! (2008), and Yuxie's Shadow! (2009), respectively.
Pokémon Hunter J succeeds in capturing the lake guardians and hands them over to Team Galactic.
Despite sharing the same name in both Japanese and English, Uxie has two different voice actors for both languages.
The lake guardians are placed under Cyrus's control, but they manage to teleport Ash, Brock, and Dawn to the Team Galactic HQ before being completely subdued.
During this episode, both Mesprit and Uxie use Teleport, a move neither can legally learn.