(at around 4 mins) In the first scene you see a mansion identical to the Spencer Mansion in the first game, including the main hall and stairway.
In some scenes, Rebecca is wearing the same medic uniform she wore in Resident Evils, 0, and 1 (both versions).
Features the first animated story appearance of Rebecca Chambers, her last in-game appearance before this film was Resident Evil Zero (2002). First released 15 years earlier.
(at around 26 mins) The first zombie Rebecca sees is eating a dead victim's body, then looks back over his shoulder at Rebecca. This happened with the first zombie in both the original and remake Resident Evil game where it ate a victim, then looked back over its shoulder back either Chris or Jill.
This is the first Resident Evil game-canon title to take place in real world cities, like Chicago and New York City.